URINE PRESERVATIVES - Clínica Mayo

Prévia do material em texto

Urine Preservatives—Collection and Transportation for 24-Hour Urine Specimens 
Page 1 of 6 04/24/2012 
 
The Department of Laboratory Medicine and Pathology at Mayo Clinic initiated a study to determine stability of analytes under different preservative conditions. This 
reference chart, built from that study, will enable you to select either temperature conditions or preservatives to maximize the number of tests that can be performed on 
a single 24-hour collection. 
 
Note: See the Test Catalog for test-specific collection requirements. 
 
 
 PRESERVATIVE OPTIONS TRANSPORT TEMPERATURE 
Published Test 
Name 
Unit Code/ 
Mnemonic 
External Only 
Boric Acid 
 
50% 
Acetic 
Acid 
Toluene 
 
Na(2)CO(3) 
 
6N HCL 
 
6N 
HN0(3) 
 
Thymol 
 
Ambient 
(Plain) 
Internal Only External Internal Ambient Refrigerate Frozen 
Aldosterone, Urine 8556/ALDU OK OK OK OK Preferred OK No No No OK No 
Refrig Preferred
Frozen OK 
Ambient w/preservative 
OK
Ambient OK 
Aldosterone with Sodium, 
Urine 15150/ALD/NA OK OK OK OK Preferred OK No No No OK No 
Refrig Preferred
Frozen OK 
Ambient w/preservative 
OK
Ambient OK 
Alpha-1-Microglobulin, 24 
Hour, Urine 81036/A1M24 Preferred OK OK OK OK OK No No No OK Preferred 
Refrig Preferred
Ambient OK 
Frozen OK
Ambient 
Preferred 
Aluminum, 24 Hour, Urine 8828/ALU OK Preferred OK No OK OK No OK OK No OK 
Refrig Preferred
Frozen OK 
Ambient OK
Ambient OK 
Aminolevulinic Acid (ALA), 
Urine 8406/DALA No No No No Preferred No No OK No No No 
Frozen Preferred
Refrig OK 
Ambient NO
On Wet Ice 
Antimony 8871/ZW16 OK OK OK No No OK No Preferred OK No No 
Ambient Preferred
Refrig OK 
Frozen OK
Ambient OK 
Arsenic, 24 Hour, Urine 8644/ASU OK Preferred OK No OK OK No OK No No OK 
Refrig Preferred
Frozen OK 
Ambient OK
Ambient OK 
Arsenic Fractionation, 24 
Hour, Urine 80375/ASFR OK Preferred OK No OK OK No OK No No OK 
Refrig Preferred
Frozen OK 
Ambient OK
Ambient OK 
Arylsulfatase A, Urine 8777/ARSU No Required No No No No No No No No No 
Refrig Preferred
Ambient NO 
Frozen NO
Refrig OK 
Ambient NO 
Frozen NO 
Beryllium 8871/ZW16 OK OK OK No OK OK No Preferred OK No No 
Refrig Preferred
Frozen OK 
Ambient NO
Ambient OK 
6-Beta-
Hydroxycortisol/Cortisol 
Ratio, Free, Urine 
85533/CORT6 OK OK Preferred OK OK No No No No No OK 
Frozen Preferred
Refrig OK 
Ambient OK
Ambient OK 
11 Beta-Prostaglandin F2 
Alpha, Urine 81425/BPG2 No OK OK OK Preferred OK No OK OK OK No 
Frozen Preferred
Refrig OK 
Ambient NO
Refrig OK 
Ambient NO 
Urine Preservatives—Collection and Transportation for 24-Hour Urine Specimens 
Page 2 of 6 04/24/2012 
 PRESERVATIVE OPTIONS TRANSPORT TEMPERATURE 
Published Test 
Name 
Unit Code/ 
Mnemonic 
External Only 
Boric Acid 
 
50% 
Acetic 
Acid 
Toluene 
 
Na(2)CO(3) 
 
6N HCL 
 
6N 
HN0(3) 
 
Thymol 
 
Ambient 
(Plain) 
Internal Only External Internal Ambient Refrigerate Frozen 
Bismuth 8871/ZW16 OK OK No No No No No Preferred OK No No 
Refrig w/ preservative 
Preferred 
Ambient NO 
Frozen NO
Ambient OK 
Cadmium, 24 Hour, Urine 8678/CDU OK Preferred OK No OK OK No OK OK No No 
Refrig Preferred
Frozen OK 
Ambient NO
Ambient OK 
Calcium, 24 Hour, Urine 8594/CAU OK Preferred OK OK OK OK No OK OK No OK 
Refrig Preferred
Frozen OK 
Ambient NO
Ambient OK 
Calcium/Creatinine, 24 Hour, 
Urine 89777/CACU OK Preferred OK OK OK OK No OK OK No OK 
Refrig Preferred
Frozen OK 
Ambient OK 
Ambient OK 
Catecholamine Fractionation, 
Free, Urine 9276/CATU No OK OK OK Preferred No No OK OK OK No 
Refrig Preferred
Ambient w/ preservative 
OK 
Frozen OK
Ambient OK 
Chloride, Urine 8531/CLU OK Preferred OK OK OK OK No No No OK Preferred 
Refrig Preferred
Frozen OK 
Ambient NO
Ambient OK 
Chromium, 24 Hour, Urine 8593/CRU OK Preferred OK No No No No No No No No 
Refrig Preferred
Frozen OK 
Ambient OK
Ambient OK 
Citrate Excretion, Urine 9329/CITR No OK OK Preferred OK OK No OK OK OK No 
Refrig Preferred
Frozen <7 days OK 
Ambient NO
Ambient OK 
Cobalt, 24 Hour, Urine 80083/COU OK Preferred OK No OK OK No OK OK No OK 
Refrig Preferred
Frozen OK 
Ambient OK
Ambient OK 
Copper, 24 Hour, Urine 8590/CUU No Preferred OK No OK OK No OK OK No No 
Refrig Preferred
Frozen OK 
Ambient NO
Refrig OK 
Ambient NO 
Cortisol/Cortisone, Free, 
Urine 82948/COCOU No OK OK Preferred OK OK OK No No OK No 
Refrig
Preferred 
Frozen OK 
Ambient w/preservative 
OK
Ambient OK 
Cortisol, Free, Urine 8546/CORTU No OK OK Preferred OK OK OK No No OK No 
Refrig Preferred 
Frozen OK 
Ambient w/preservative 
OK
Ambient OK 
Creatinine, Urine 
 8513/CTU Preferred OK OK OK OK OK OK OK OK OK Preferred 
Frozen <7 days Preferred
Refrig <7 days OK 
Ambient <7 days OK
Ambient OK 
Creatinine Clearance 8500/CRC Preferred OK OK OK OK OK OK OK OK OK Preferred 
Refrig Preferred
Frozen OK 
Ambient OK
Ambient OK 
Urine Preservatives—Collection and Transportation for 24-Hour Urine Specimens 
Page 3 of 6 04/24/2012 
 PRESERVATIVE OPTIONS TRANSPORT TEMPERATURE 
Published Test 
Name 
Unit Code/ 
Mnemonic 
External Only 
Boric Acid 
 
50% 
Acetic 
Acid 
Toluene 
 
Na(2)CO(3) 
 
6N HCL 
 
6N 
HN0(3) 
 
Thymol 
 
Ambient 
(Plain) 
Internal Only External Internal Ambient Refrigerate Frozen 
Cystinuria Profile, 
Quantitative, 24 Hour, Urine 8376/CYSQN No OK OK No No Preferred No No No No No 
Frozen Preferred
Refrig with Toluene OK 
Ambient NO
Ambient OK 
Electrophoresis, Protein, 
Urine 82441/EPU OK Preferred OK No No OK No No No OK Preferred 
Refrig Preferred
Frozen OK 
Ambient <3 days OK
Ambient OK 
Electrophoresis, Protein, 
Urine 
INTERNAL ONLY 
8244/PEU OK Preferred OK No No OK No No No No Preferred 
Refrig Preferred
Frozen OK 
Ambient <3 days OK
Ambient OK 
Gadolinium, 24 Hour, Urine 89301/GDU OK Preferred OK No No OK No OK OK No OK 
Refrig Preferred
Frozen OK 
Ambient OK
Ambient OK 
Glucose, Urine 8412/GLUR NO OK OK Preferred OK OK No OK OK OK No 
Refrig <7 days Preferred
Frozen <7 days OK 
Ambient NO
Ambient OK 
Gold 8871/ZW16 OK Preferred OK No OK OK No OK OK No No 
Refrig Preferred
Frozen OK 
Ambient NO
Ambient OK 
Heavy Metals Screen, 24 
Hour, Urine 8633/HMSU No Preferred OK No OK OK No OK No No No 
Refrig Preferred
Ambient OK 
Frozen OK
Ambient OK 
Homovanillic Acid (HVA), 24 
Hour, Urine 9253/HVA No No No No Preferred No No OK No No No 
Refrig Preferred
Frozen OK 
Ambient NO
Ambient OK 
5-Hydroxyindoleacetic Acid 
(5-HIAA), Urine 9248/HIAA No OK OK OK Preferred OK OK OK OK OK No 
Refrig Preferred
Frozen OK 
Ambient NO
Ambient OK 
Immunofixation, Urine 82025/IFXU OK Preferred OK No No OK No No No OK Preferred 
Refrig Preferred
Frozen OK 
Ambient NO
Ambient OK 
Immunoglobulin Total Light 
Chains, Urine 87934/TLCU 
YES <72 
Hours Preferred OK No No No No No No No <72 Hours OK
Refrig <14 days Preferred 
Frozen <20 days OK 
Ambient <72 Hours OK 
 
Inherited Conjugated 
Hyperbilirubinemias, Urine 8652/CI No No No No No No Required No No No No 
Frozen Preferred
Refrig NO 
Ambient NO
Ambient OK 
Iodine, 24 Hour, Urine 9549/UIOD OK Preferred OK No OK No No No No No OK 
Refrig Preferred
Ambient OK 
Frozen OK
Ambient OK 
Iron, 24 Hour, Urine 8571/FEU No Preferred OK No OK OK No OK OK No No 
Refrig Preferred
Frozen OK 
Ambient NO
Ambient OK 
Lead, 24 Hour, Urine 8600/PBU OK Preferred OK No OK OK No OK OK No OK 
Refrig Preferred 
Frozen OK 
Ambient NO
Ambient OK 
Magnesium, 24 Hour, Urine 8595/MGU OK Preferred OK OK OK OK No OK OK No OK 
Refrig Preferred
Frozen OK 
Ambient NO
Ambient OK 
Urine Preservatives—Collection and Transportation for 24-Hour Urine Specimens 
Page 4 of 6 04/24/2012 
 PRESERVATIVE OPTIONS TRANSPORT TEMPERATUREPublished Test 
Name 
Unit Code/ 
Mnemonic 
External Only 
Boric Acid 
 
50% 
Acetic 
Acid 
Toluene 
 
Na(2)CO(3) 
 
6N HCL 
 
6N 
HN0(3) 
 
Thymol 
 
Ambient 
(Plain) 
Internal Only External Internal Ambient Refrigerate Frozen 
Manganese, 24 Hour, Urine 8080/MNU OK Preferred OK No No No No No No No OK 
Refrig Preferred
Frozen OK 
Ambient OK
Ambient OK 
Mercury, 24 Hour, Urine 8592/HGU No Preferred OK No OK OK No OK OK No No 
Refrig Preferred
Frozen OK 
Ambient NO
Ambient OK 
Metanephrines, Fractionated, 
24 Hour, Urine 83006/METAF No OK OK Preferred Preferred OK OK OK OK OK OK 
Refrig Preferred
Frozen OK 
Ambient OK
Ambient OK 
N-Methylhistamine, Urine 83011/NMHIN OK Preferred OK OK OK OK OK OK OK OK OK 
Refrig Preferred
Frozen OK 
Ambient OK
Ambient OK 
Microalbuminuria, 24-Hour, 
Urine 9386/MA24 Preferred OK OK OK No OK No No No OK OK 
Refrig Preferred
Frozen < or =7 days OK 
Ambient < or =7 days OK
Ambient OK 
Molybdenum, 24-Hour, Urine 89271/MOLU OK Preferred OK No OK OK No No No No OK 
 Refrig Preferred 
Ambient OK 
Frozen OK
Ambient OK 
Monoclonal Protein Study, 
Urine 8823/MPSU OK Preferred OK No No OK No No No OK Preferred 
Refrig Preferred
Frozen OK 
Ambient <3 days OK
Ambient OK 
Nickel, 24 Hour, Urine 8626/NIU OK Preferred No No OK No No No OK No OK 
Refrig Preferred
Frozen OK 
Ambient NO
Ambient OK 
Nitrogen, Total, Urine 8586/NITU OK OK Preferred No OK OK OK OK No OK Preferred 
Frozen Preferred
Refrig OK 
Ambient OK
Ambient OK 
NTX-Telopeptide, Urine 81549/NTXP No OK <5 days Preferred OK No OK No No No No No 
Frozen Preferred
Refrig <5 days OK 
Ambient NO
Ambient OK 
Oxalate, Urine 8669/OXU OK OK OK OK OK OK No Preferred OK OK No 
Refrig Preferred
Frozen <7 days OK 
Ambient NO
Ambient OK 
Phosphorus, Urine 8526/POU OK Preferred OK OK OK OK No OK OK OK Preferred 
Refrig Preferred
Frozen <7 days OK 
Ambient <7 days OK
Ambient OK 
pH, 24 Hour, Urine 84047/UPH24 No OK OK No No Preferred No No No OK No 
Refrig <7 days Preferred 
Frozen <7 days OK 
Ambient NO
Ambient OK 
Porphyrins, Quantitative, 
Urine 8562/PQNU No No No No No No Required No No No No 
Frozen Preferred
Refrig NO 
Ambient NO
Ambient OK 
Protect from 
Light
Potassium, Urine 8527/KUR OK Preferred OK OK OK OK No OK OK OK Preferred 
Refrig Preferred
Frozen OK 
Ambient NO
Ambient OK 
Protein, Total, Urine 8261/PTU Preferred OK OK OK No OK OK No No OK Preferred 
Refrig Preferred
Frozen <7 days OK 
Ambient <7 days OK
Ambient OK 
Urine Preservatives—Collection and Transportation for 24-Hour Urine Specimens 
Page 5 of 6 04/24/2012 
 PRESERVATIVE OPTIONS TRANSPORT TEMPERATURE 
Published Test 
Name 
Unit Code/ 
Mnemonic 
External Only 
Boric Acid 
 
50% 
Acetic 
Acid 
Toluene 
 
Na(2)CO(3) 
 
6N HCL 
 
6N 
HN0(3) 
 
Thymol 
 
Ambient 
(Plain) 
Internal Only External Internal Ambient Refrigerate Frozen 
Retinol-Binding Protein, 24 
Hour, Urine 81783/RBP24 Preferred OK OK OK No OK No No No OK Preferred 
Refrig Preferred
Frozen < or =7 days OK 
Ambient NO
Ambient 
Preferred 
Selenium, 24 Hour, Urine 9793/SEU OK OK Preferred No OK OK No OK OK No OK 
Frozen Preferred
Refrig OK 
Ambient OK
Ambient OK 
Serotonin, 24 Hour, Urine 87834/SERU No Preferred OK No Preferred OK No No OK OK No 
Frozen Preferred
Refrig <7 days OK 
Ambient NO
Frozen 
Sodium, Urine 8525/NAU OK Preferred OK OK OK OK No OK OK OK Preferred 
Refrig <7 days Preferred
Frozen <7 days OK 
Ambient <7 days OK
Ambient OK 
Sulfate, Urine 81815/SULFU No Preferred OK OK No OK OK No No OK Preferred 
Refrig Preferred
Frozen OK 
Ambient NO
Ambient OK 
Supersaturation Profile, 
Urine 82029/SSAT No OK OK No No Preferred No No No No No 
Refrig Preferred
Frozen < 7 days OK 
Ambient NO
Ambient OK 
Thallium, 24 Hour, Urine 8603/TLU OK Preferred OK No OK OK No OK OK No OK 
Refrig Preferred
Frozen OK 
Ambient OK
Ambient OK 
Tin 8871/ZW16 OK Preferred OK No OK No No OK OK No No 
Refrig Preferred
Frozen OK 
Ambient NO
Ambient OK 
Urea, Urine 8330/URAU No OK OK Preferred OK OK OK OK OK OK No 
Refrig Preferred
Frozen OK 
Ambient NO
Ambient OK 
Uric Acid, Urine 8529/URCU No OK OK Preferred No OK OK No No OK No 
Refrig Preferred
Frozen < 7 days OK 
Ambient NO
Ambient OK 
Vanadium, 24 Hour, Urine 83395/VU OK Preferred OK No No No No No No No OK 
Refrig Preferred
Frozen OK 
Ambient OK
Ambient OK 
Vanillylmandelic Acid (VMA), 
24 Hour, Urine 9454/VMA No No No OK Preferred No No OK OK No No 
Refrig Preferred
Frozen OK 
Ambient NO
Ambient OK 
Xanthine and Hypoxanthine, 
Urine 80313/XAN No OK Preferred No No No No No No No No 
Frozen Preferred
Refrig OK 
Ambient NO
Ambient OK 
Zinc, 24 Hour, Urine 8591/ZNU No Preferred OK No OK OK No OK OK No No 
Refrig Preferred
Frozen OK 
Ambient NO
Ambient OK 
 
 
Urine Preservatives—Collection and Transportation for 24-Hour Urine Specimens 
Page 6 of 6 04/24/2012 
The conditions and concentrations of urine preservatives are defined for 24-hour collections as follows: 
 
Ambient Room temperature (21degrees - 25 degrees C) 
Refrigerated 4 degrees C 
Frozen -20 degrees C 
6N HCl 30 mL per 24-hour collection 
50% Acetic Acid 25 mL per 24-hour collection 
Na2CO3 (crystals) 5 g per 24-hour collection 
Toluene 30 mL per 24-hour collection 
6N HNO3 15 mL per 24-hour collection 
Boric Acid (crystals) 10 g per 24-hour collection 
Thymol (10% in isopropanol) 10 mL per 24-hour collection 
 
 
Preservative and condition guidelines have been evaluated specifically for Mayo Medical Laboratories assays. If you have questions regarding the use of 
preservatives, contact Mayo Medical Laboratories at 800-533-1710 or 507-266-5700.